Ontology highlight
ABSTRACT:
SUBMITTER: Yang J
PROVIDER: S-EPMC2705776 | biostudies-literature | 2009
REPOSITORIES: biostudies-literature
Yang Jinfu J Tang Tao T Li Feng F Zhou Wenwu W Liu Jian J Tan Zhiping Z Zheng Wei W Yang Yifeng Y Zhou Xinmin X Hu Jianguo J
Journal of biomedicine & biotechnology 20090701
<h4>Objective</h4>To construct the eukaryotic expression vector hypoxia-inducible factor 1alpha-pcDNA(3.1) and to investigate its transfective efficiency into mesenchymal stem cells (MSCs) in vitro and the expression of HIF-1alpha gene in MSCs.<h4>Methods</h4>mRNA of Wistar Rats' myocardial cells was extracted, and cDNA was synthesized with Reverse Transcription Kit, HIF-1alpha was amplified by polymerase chain reaction (PCR), and constructed into pcDNA(3.1). Transfected HIF-1alpha-pcDNA(3.1) in ...[more]