Ontology highlight
ABSTRACT:
SUBMITTER: Kwon T
PROVIDER: S-EPMC2707113 | biostudies-literature | 2009
REPOSITORIES: biostudies-literature
Kwon Taeyun T Park Jinsung J Yang Jaemoon J Yoon Dae Sung DS Na Sungsoo S Kim Chang-Wan CW Suh Jin-Suck JS Huh Yong-Min YM Haam Seungjoo S Eom Kilho K
PloS one 20090716 7
Characterization and control of proteolysis of peptides by specific cellular protease is a priori requisite for effective drug discovery. Here, we report the nanomechanical, in situ monitoring of proteolysis of peptide chain attributed to protease (Cathepsin B) by using a resonant nanomechanical microcantilever immersed in a liquid. Specifically, the detection is based on measurement of resonant frequency shift arising from proteolysis of peptides (leading to decrease of cantilever's overall mas ...[more]