Transcriptome sequencing of the Microarray Quality Control (MAQC) RNA reference samples using next generation sequencing.
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ABSTRACT:
SUBMITTER: Mane SP
PROVIDER: S-EPMC2707382 | biostudies-literature | 2009
REPOSITORIES: biostudies-literature
Publications
Transcriptome sequencing of the Microarray Quality Control (MAQC) RNA reference samples using next generation sequencing.
Mane Shrinivasrao P SP Evans Clive C Cooper Kristal L KL Crasta Oswald R OR Folkerts Otto O Hutchison Stephen K SK Harkins Timothy T TT Thierry-Mieg Danielle D Thierry-Mieg Jean J Jensen Roderick V RV
BMC genomics 20090612
<h4>Background</h4>Transcriptome sequencing using next-generation sequencing platforms will soon be competing with DNA microarray technologies for global gene expression analysis. As a preliminary evaluation of these promising technologies, we performed deep sequencing of cDNA synthesized from the Microarray Quality Control (MAQC) reference RNA samples using Roche's 454 Genome Sequencer FLX.<h4>Results</h4>We generated more that 3.6 million sequence reads of average length 250 bp for the MAQC A ...[more]
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