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Proteomic identification of small, copper-responsive proteins in germinating embryos of Oryza sativa.


ABSTRACT: BACKGROUND AND AIMS: Although copper (Cu) is an essential micronutrient for plants and algae, excess Cu is toxic to most plants and can cause a wide range of deleterious effects. To investigate the response of rice (Oryza sativa) to Cu stress, a proteomic approach was used to analyse Cu stress-induced changes in the expression of low molecular-weight proteins in germinating rice seed embryos. METHODS: Rice seeds were germinated in the presence or absence of 200 microm Cu for 6 d, and embryos, including newly formed shoots and radicles, were isolated. After proteins were extracted from the germinating embryos and separated by two-dimensional PAGE, 16 proteins in the 6- to 25-kDa range were identified using MALDI-TOF mass spectrometry. KEY RESULTS AND CONCLUSIONS: Thirteen of the proteins identified, including metallothionein-like protein, membrane-associated protein-like protein, putative wall-associated protein kinase, pathogenesis-related proteins and the putative small GTP-binding protein Rab2, were up-regulated by Cu stress. Three proteins, a putative small cytochrome P450 (CYP90D2), a putative thioredoxin and a putative GTPase, were down-regulated by Cu stress. As far as is known, this study provides the first proteomic evidence that metallothionein and CYP90D2 are Cu-responsive proteins in plants. These findings may lead to a better understanding of plant molecular responses to toxic metal exposure.

SUBMITTER: Zhang H 

PROVIDER: S-EPMC2707895 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature

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Proteomic identification of small, copper-responsive proteins in germinating embryos of Oryza sativa.

Zhang Hongxiao H   Lian Chunlan C   Shen Zhenguo Z  

Annals of botany. 20090205 6


<h4>Background and aims</h4>Although copper (Cu) is an essential micronutrient for plants and algae, excess Cu is toxic to most plants and can cause a wide range of deleterious effects. To investigate the response of rice (Oryza sativa) to Cu stress, a proteomic approach was used to analyse Cu stress-induced changes in the expression of low molecular-weight proteins in germinating rice seed embryos.<h4>Methods</h4>Rice seeds were germinated in the presence or absence of 200 microm Cu for 6 d, an  ...[more]

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