Pch2 links chromosome axis remodeling at future crossover sites and crossover distribution during yeast meiosis.

ABSTRACT: Segregation of homologous chromosomes during meiosis I depends on appropriately positioned crossovers/chiasmata. Crossover assurance ensures at least one crossover per homolog pair, while interference reduces double crossovers. Here, we have investigated the interplay between chromosome axis morphogenesis and non-random crossover placement. We demonstrate that chromosome axes are structurally modified at future crossover sites as indicated by correspondence between crossover designation marker Zip3 and domains enriched for axis ensemble Hop1/Red1. This association is first detected at the zygotene stage, persists until double Holliday junction resolution, and is controlled by the conserved AAA+ ATPase Pch2. Pch2 further mediates crossover interference, although it is dispensable for crosso

SUBMITTER: Joshi N 

PROVIDER: S-EPMC2708914 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature