Ontology highlight
ABSTRACT:
SUBMITTER: Akopiants K
PROVIDER: S-EPMC2709571 | biostudies-literature | 2009 Jul
REPOSITORIES: biostudies-literature
Akopiants Konstantin K Zhou Rui-Zhe RZ Mohapatra Susovan S Valerie Kristoffer K Lees-Miller Susan P SP Lee Kyung-Jong KJ Chen David J DJ Revy Patrick P de Villartay Jean-Pierre JP Povirk Lawrence F LF
Nucleic acids research 20090506 12
XLF/Cernunnos is a core protein of the nonhomologous end-joining pathway of DNA double-strand break repair. To better define the role of Cernunnos in end joining, whole-cell extracts were prepared from Cernunnos-deficient human cells. These extracts effected little joining of DNA ends with cohesive 5' or 3' overhangs, and no joining at all of partially complementary 3' overhangs that required gap filling prior to ligation. Assays in which gap-filled but unligated intermediates were trapped using ...[more]