Ontology highlight
ABSTRACT:
SUBMITTER: Lee JH
PROVIDER: S-EPMC2709985 | biostudies-literature | 2009 Jun
REPOSITORIES: biostudies-literature
Lee Jin-Hee JH Evans Bradley S BS Li Gongyong G Kelleher Neil L NL van der Donk Wilfred A WA
Biochemistry 20090601 23
The late stages of biosynthesis of phosphinothricin tripeptide (PTT) involve peptide formation and methylation on phosphorus. The exact timing of these transformations is not known. To provide insight into this question, we developed a heterologous expression system for PhsA, one of three NRPS proteins in PTT biosynthesis. The apparent k(cat)/K(m) value for ATP-pyrophosphate exchange activity for d,l-N-acetylphosphinothricin was 3.5 muM(-1) min(-1), whereas the k(cat)/K(m,app) for l-N-acetyldeme ...[more]