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In vivo labeling method using a genetic construct for nanoscale resolution microscopy.

ABSTRACT: We demonstrate beam scanning-stimulated emission depletion microscopy with in vivo labeled cells. A red emitting fluorescent dye is introduced into membrane protein fused to a multifunctional reporter protein (HaloTag, Promega, Madison, WI) in live cells. This approach allows superresolution stimulated emission depletion imaging without the limitations of immunofluorescence-based staining.

SUBMITTER: Schroder J

PROVIDER: S-EPMC2710050 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature

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In vivo labeling method using a genetic construct for nanoscale resolution microscopy.

Schröder Jan J Benink Hélène H Dyba Marcus M Los Georgyi V GV

Biophysical journal 20090101 1

We demonstrate beam scanning-stimulated emission depletion microscopy with in vivo labeled cells. A red emitting fluorescent dye is introduced into membrane protein fused to a multifunctional reporter protein (HaloTag, Promega, Madison, WI) in live cells. This approach allows superresolution stimulated emission depletion imaging without the limitations of immunofluorescence-based staining. ...[more]

PMID: 19134467

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