Mitochondrial haplogroups and control region polymorphisms are not associated with prostate cancer in Middle European Caucasians.
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ABSTRACT: Background
Besides being responsible for energy production in the cell, mitochondria are central players in apoptosis as well as the main source of harmful reactive oxygen species. Therefore, it can be hypothesised that sequence variation in the mitochondrial genome is a contributing factor to the etiology of diseases related to these different cellular events, including cancer. The aim of the present study was to assess the frequency of haplogroups and polymorphisms in the control region (CR) of mitochondrial DNA of peripheral blood mononuclear cells from patients with prostate carcinoma (n = 304) versus patients screened for prostate disease but found to be negative for cancer on biopsy (n = 278) in a Middle European population.Methodology/principal findings
The nine major European haplogroups and the CR polymorphisms were identified by means of primer extension analysis and DNA sequencing, respectively. We found that mitochondrial haplogroup frequencies and CR polymorphisms do not differ significantly between patients with or without prostate cancer, implying no impact of inherited mitochondrial DNA variation on predisposition to prostate carcinoma in a Middle European population.Conclusions/significance
Our results contrast with a recent report claiming an association between mtDNA haplogroup U and prostate cancer in a North American population of caucasian descent.
SUBMITTER: Mueller EE
PROVIDER: S-EPMC2712094 | biostudies-literature |
REPOSITORIES: biostudies-literature
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| S-EPMC3027676 | biostudies-literature
Project description:BackgroundMitochondrial DNA (mtDNA) haplogroups have been associated with advanced liver fibrosis and cirrhosis in patients coinfected with human immunodeficiency virus (HIV) and hepatitis C virus (HCV). Our aim was to determine whether mtDNA haplogroups are associated with liver-related events (LREs) in HIV/HCV-coinfected patients.MethodsWe carried out a retrospective cohort study in HIV/HCV-coinfected patients who were potential candidates for therapy with interferon and ribavirin (IFN/Rib) between 2000 and 2009. The primary endpoint was the occurrence of LREs (decompensation or hepatocellular carcinoma). mtDNA genotyping was performed using the Sequenom MassARRAY platform. We used Fine and Gray proportional hazards model to test the association between mtDNA haplogroups and LREs, considering death as a competitive risk.ResultsThe study population comprised 243 patients, of whom 40 had advanced fibrosis or cirrhosis. After a median follow-up of 7.7 years, 90 patients treated with IFN/Rib achieved sustained viral response (SVR), 18 patients had LREs, and 11 patients died. Patients with haplogroup H had lower cumulative incidence than patients with other haplogroups (p?=?0.012). However, patients with haplogroup T had higher cumulative incidence than patients with other haplogroups (p?=?0.074). In the multivariate analysis, haplogroup T was associated with an increased hazard of developing LREs [adjusted subhazard ratio (aSHR)?=?3.56 (95% CI 1.13;11.30); p?=?0.030]; whereas haplogroup H was not associated with lower hazard of LREs [aSHR?=?0.36 (95% CI 0.10;1.25); p?=?0.105]. When we excluded patients who achieved SVR during follow-up, we obtained similar SHR values.ConclusionsEuropean mitochondrial haplogroups may influence the natural history of chronic hepatitis C.
| S-EPMC6660654 | biostudies-literature