Detection of RASSF1A promoter hypermethylation in serum from gastric and colorectal adenocarcinoma patients.

ABSTRACT:

Aim

To evaluate the diagnostic role of serum RASSF1A promoter hypermethylation in gastric and colorectal adenocarcinoma.

Methods

Methylation-specific polymerase chain reaction (MSPCR) was used to examine the promoter methylation status of the serum RASSF1A gene in 47 gastric adenocarcinoma patients, 45 colorectal adenocarcinoma patients, 60 patients with benign gastrointestinal disease (30 with benign gastric disease and 30 with benign colorectal disease), and 30 healthy donor controls. A paired study of RASSF1A promoter methylation status in primary tumor, adjacent normal tissue, and postoperative serum were conducted in 25 gastric and colorectal adenocarcinoma patients who later were underwent surgical therapy.

Results

The frequencies of detection of serum RASSF1A p

SUBMITTER: Wang YC 

PROVIDER: S-EPMC2712178 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature