Ontology highlight
ABSTRACT:
SUBMITTER: Banks DD
PROVIDER: S-EPMC2712195 | biostudies-literature | 2009 May
REPOSITORIES: biostudies-literature
Banks Douglas D DD Scavezze Joanna L JL Siska Christine C CC
Biophysical journal 20090501 10
This report describes what to our knowledge is the first kinetic folding studies of erythropoietin, a glycosylated four-helical bundle cytokine responsible for the regulation of red blood cell production. Kinetic responses for folding and unfolding reactions initiated by manual mixing were monitored by far-ultraviolet circular dichroism and fluorescence spectroscopy, and folding reactions initiated by stopped-flow mixing were monitored by fluorescence. The urea concentration dependence of the ob ...[more]