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Multiplexed DNA detection using spectrally encoded porous SiO2 photonic crystal particles.

ABSTRACT: A particle-based multiplexed DNA assay based on encoded porous SiO(2) photonic crystal disks is demonstrated. A "spectral barcode" is generated by electrochemical etch of a single-crystal silicon wafer using a programmed current-time waveform. A lithographic procedure is used to isolate cylindrical microparticles 25 microm in diameter and 10 microm thick, which are then oxidized, modified with a silane linker, and conjugated to various amino-functionalized oligonucleotide probes via cyanuric chloride. It is shown that the particles can be decoded based on their reflectivity spectra and that a multiple analyte assay can be performed in a single sample with a modified fluorescence microscope. The homogeneity of the reflectivity and fluorescence spectra, both within and across the microparticles, is also reported.

SUBMITTER: Meade SO

PROVIDER: S-EPMC2713104 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature

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Multiplexed DNA detection using spectrally encoded porous SiO2 photonic crystal particles.

Meade Shawn O SO Chen Michelle Y MY Sailor Michael J MJ Miskelly Gordon M GM

Analytical chemistry 20090401 7

A particle-based multiplexed DNA assay based on encoded porous SiO(2) photonic crystal disks is demonstrated. A "spectral barcode" is generated by electrochemical etch of a single-crystal silicon wafer using a programmed current-time waveform. A lithographic procedure is used to isolate cylindrical microparticles 25 microm in diameter and 10 microm thick, which are then oxidized, modified with a silane linker, and conjugated to various amino-functionalized oligonucleotide probes via cyanuric chl ...[more]

PMID: 19271746

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