Polyvalent display of heme on hepatitis B virus capsid protein through coordination to hexahistidine tags.
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ABSTRACT:
SUBMITTER: Prasuhn DE
PROVIDER: S-EPMC2715361 | biostudies-literature | 2008 May
REPOSITORIES: biostudies-literature
Publications
Polyvalent display of heme on hepatitis B virus capsid protein through coordination to hexahistidine tags.
Prasuhn Duane E DE Kuzelka Jane J Strable Erica E Udit Andrew K AK Cho So-Hye SH Lander Gabriel C GC Quispe Joel D JD Diers James R JR Bocian David F DF Potter Clint C Carragher Bridget B Finn M G MG
Chemistry & biology 20080501 5
The addition of a hexahistidine tag to the N terminus of the hepatitis B capsid protein gives rise to a self-assembled particle with 80 sites of high local density of histidine side chains. Iron protoporphyrin IX has been found to bind tightly at each of these sites, making a polyvalent system of well-defined spacing between metalloporphyrin complexes. The spectroscopic and redox properties of the resulting particle are consistent with the presence of 80 site-isolated bis(histidine)-bound heme c ...[more]
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