Ontology highlight
ABSTRACT:
SUBMITTER: Pavoor TV
PROVIDER: S-EPMC2715507 | biostudies-literature | 2009 Jul
REPOSITORIES: biostudies-literature
Pavoor Tej V TV Cho Yong Ku YK Shusta Eric V EV
Proceedings of the National Academy of Sciences of the United States of America 20090702 29
Proteins that can bind specifically to targets that also have an intrinsic property allowing for easy detection could facilitate a multitude of applications. While the widely used green fluorescent protein (GFP) allows for easy detection, attempts to insert multiple binding loops into GFP to impart affinity for a specific target have been met with limited success because of the structural sensitivity of the GFP chromophore. In this study, directed evolution using a surrogate loop approach and ye ...[more]