Label-free in situ imaging of lignification in the cell wall of low lignin transgenic Populus trichocarpa.
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ABSTRACT:
SUBMITTER: Schmidt M
PROVIDER: S-EPMC2715566 | biostudies-literature | 2009 Aug
REPOSITORIES: biostudies-literature
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Label-free in situ imaging of lignification in the cell wall of low lignin transgenic Populus trichocarpa.
Schmidt M M Schwartzberg A M AM Perera P N PN Weber-Bargioni A A Carroll A A Sarkar P P Bosneaga E E Urban J J JJ Song J J Balakshin M Y MY Capanema E A EA Auer M M Adams P D PD Chiang V L VL Schuck P James PJ
Planta 20090613 3
Chemical imaging by confocal Raman microscopy has been used for the visualization of the cellulose and lignin distribution in wood cell walls. Lignin reduction in wood can be achieved by, for example, transgenic suppression of a monolignol biosynthesis gene encoding 4-coumarate-CoA ligase (4CL). Here, we use confocal Raman microscopy to compare lignification in wild type and lignin-reduced 4CL transgenic Populus trichocarpa stem wood with spatial resolution that is sub-microm. Analyzing the lign ...[more]
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