The first virally encoded cytochrome p450.
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ABSTRACT:
SUBMITTER: Lamb DC
PROVIDER: S-EPMC2715754 | biostudies-literature | 2009 Aug
REPOSITORIES: biostudies-literature
Publications
The first virally encoded cytochrome p450.
Lamb David C DC Lei Li L Warrilow Andrew G S AG Lepesheva Galina I GI Mullins Jonathan G L JG Waterman Michael R MR Kelly Steven L SL
Journal of virology 20090610 16
The genome sequence of the giant virus Acanthamoeba polyphaga mimivirus revealed the presence of two putative cytochrome P450 (CYP) genes. The product of one of the two predicted CYP genes (YP_143162) showed low-level homology to sterol 14-demethylase (CYP51) and contained a C-terminal polypeptide domain of unknown function. YP_143162 expression (without an N-terminal membrane binding domain) in Escherichia coli yields a CYP protein which gives a reduced CO difference maximum at 448 nm and was f ...[more]
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