Ontology highlight
ABSTRACT:
SUBMITTER: Conley NR
PROVIDER: S-EPMC2715847 | biostudies-literature | 2008 Sep
REPOSITORIES: biostudies-literature
The journal of physical chemistry. B 20080828 38
Covalent heterodimers of the Cy3 and Cy5 fluorophores have been prepared from commercially available starting materials and characterized at the single-molecule level. This system behaves as a discrete molecular photoswitch, in which photoexcitation of the Cy5 results in fluorescence emission or, with a much lower probability, causes the Cy5 to enter into a long-lived, but metastable, dark state. Photoinduced recovery of the emissive Cy5 is achieved by very low intensity excitation (5 W cm(-2)) ...[more]