Dataset Information

Molecular cloning and characterization of the complete acetylcholinesterase gene (Ace1) from the mosquito Aedes aegypti with implications for comparative genome analysis.

ABSTRACT: Insensitive acetylcholinesterase (AChE) has been shown to be responsible for resistance to organophosphates and carbamates in a number of arthropod species. Some arthropod genomes contain a single Ace gene, while others including mosquitoes contain two genes, but only one confers insecticide resistance. Here we report the isolation of the full-length cDNA and characterization of the complete genomic DNA sequence for the Ace1 gene in the yellow fever mosquito, Aedes aegypti. The Ace1 homolog in other mosquito species has been associated with insecticide resistance. The full-length cDNA consists of 2721bp and contains a 2109bp open reading frame that encodes a 702 amino acid protein. The amino acid sequence is highly conserved with that of other mosquitoes, including greater than 90% identity with Culex spp. and about 80% identity with Anopheles gambiae. The genomic DNA sequence includes 138,970bp and consists of eight exons with seven introns ranging from 59 to 114,350bp. Exons 2 and 8 show reduced amino acid conservation across mosquito species, while exons 3-7 are highly conserved. The Ace1 introns in Ae. aegypti reflect a high frequency of repetitive sequences that comprise about 45% of the total intron sequence. The Ace1 locus maps to the p-arm of chromosome 3, which corresponds to the orthologous genome regions in Culex spp. and An. gambiae.

SUBMITTER: Mori A

PROVIDER: S-EPMC2716755 | biostudies-literature | 2007 Jul

REPOSITORIES: biostudies-literature

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Molecular cloning and characterization of the complete acetylcholinesterase gene (Ace1) from the mosquito Aedes aegypti with implications for comparative genome analysis.

Mori Akio A Lobo Neil F NF deBruyn Becky B Severson David W DW

Insect biochemistry and molecular biology 20070401 7

Insensitive acetylcholinesterase (AChE) has been shown to be responsible for resistance to organophosphates and carbamates in a number of arthropod species. Some arthropod genomes contain a single Ace gene, while others including mosquitoes contain two genes, but only one confers insecticide resistance. Here we report the isolation of the full-length cDNA and characterization of the complete genomic DNA sequence for the Ace1 gene in the yellow fever mosquito, Aedes aegypti. The Ace1 homolog in o ...[more]

PMID: 17550823

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Project description:The global incidences of dengue virus have increased the interest in studying and understanding the mosquito population dynamics. It is predominantly spread by Aedes aegypti in the tropical and sub-tropical countries in the world. Understanding these dynamics is important for public health in countries where climatic and environmental conditions are favorable for the propagation of these diseases. For this reason, a new model has been proposed to investigate the population dynamics of mosquitoes in a city.The present paper discusses the numerical modeling of population dynamics of Ae. aegypti mosquitoes in an urban neighborhood of a city using the finite volume method. The model describes how populations spread through the city assisted by the wind. This model allows incorporating external factors (wind and chemical insecticides) and topography data (streets, building blocks, parks, forests and beach). The proposed model has been successfully tested in examples involving two Brazilian cities (City center, Juiz de Fora and Copacabana Beach, Rio de Janeiro).Invasion phenomena of Ae. aegypti mosquitoes have been observed in each of the simulations. It was observed that, inside the blocks, the growth of the population for both winged and aquatic phase causes an infestation of Ae. aegypti in a short time. Within the blocks the mosquito population was concentrated and diffused slowly. In the streets, there was a long-distance spread, which was influenced by wind and diffusion with a low concentration of mosquito population. The model was also tested taking into account chemical insecticides spread in two different configurations. It has been observed that the insecticides have a significant effect on the mosquito population for both winged and aquatic phases when the chemical insecticides spread more uniformly along all the streets in a neighborhood of a city.The presented methodology can be employed to evaluate and to understand the epidemic risks in a specific region of the city. Moreover the model allows an increase in efficiency of the existing mosquito population control techniques and to theoretically test new methods before involving the human population.

Dataset Information

Molecular cloning and characterization of the complete acetylcholinesterase gene (Ace1) from the mosquito Aedes aegypti with implications for comparative genome analysis.

Publications

Molecular cloning and characterization of the complete acetylcholinesterase gene (Ace1) from the mosquito Aedes aegypti with implications for comparative genome analysis.

Similar Datasets

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