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Ars2 links the nuclear cap-binding complex to RNA interference and cell proliferation.

ABSTRACT: Here we identify a component of the nuclear RNA cap-binding complex (CBC), Ars2, that is important for miRNA biogenesis and critical for cell proliferation. Unlike other components of the CBC, Ars2 expression is linked to the proliferative state of the cell. Deletion of Ars2 is developmentally lethal, and deletion in adult mice led to bone marrow failure whereas parenchymal organs composed of nonproliferating cells were unaffected. Depletion of Ars2 or CBP80 from proliferating cells impaired miRNA-mediated repression and led to alterations in primary miRNA processing in the nucleus. Ars2 depletion also reduced the levels of several miRNAs, including miR-21, let-7, and miR-155, that are implicated in cellular transformation. These findings provide evidence for a role for Ars2 in RNA interference regulation during cell proliferation.

SUBMITTER: Gruber JJ

PROVIDER: S-EPMC2717034 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature

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Ars2 links the nuclear cap-binding complex to RNA interference and cell proliferation.

Gruber Joshua J JJ Zatechka D Steven DS Sabin Leah R LR Yong Jeongsik J Lum Julian J JJ Kong Mei M Zong Wei-Xing WX Zhang Zhenxi Z Lau Chi-Kong CK Rawlings Jason J Cherry Sara S Ihle James N JN Dreyfuss Gideon G Thompson Craig B CB

Cell 20090701 2

Here we identify a component of the nuclear RNA cap-binding complex (CBC), Ars2, that is important for miRNA biogenesis and critical for cell proliferation. Unlike other components of the CBC, Ars2 expression is linked to the proliferative state of the cell. Deletion of Ars2 is developmentally lethal, and deletion in adult mice led to bone marrow failure whereas parenchymal organs composed of nonproliferating cells were unaffected. Depletion of Ars2 or CBP80 from proliferating cells impaired miR ...[more]

PMID: 19632182

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2013-11-08 | E-GEOD-52132 | biostudies-arrayexpress

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2013-11-08 | GSE52132 | GEO

Large-scale induced fit recognition of an m(7)GpppG cap analogue by the human nuclear cap-binding complex.

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