Closing gaps in the human genome using sequencing by synthesis.
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ABSTRACT:
SUBMITTER: Garber M
PROVIDER: S-EPMC2718494 | biostudies-literature | 2009
REPOSITORIES: biostudies-literature
Publications
Closing gaps in the human genome using sequencing by synthesis.
Garber Manuel M Zody Michael C MC Arachchi Harindra M HM Berlin Aaron A Gnerre Sante S Green Lisa M LM Lennon Niall N Nusbaum Chad C
Genome biology 20090602 6
The most recent release of the finished human genome contains 260 euchromatic gaps (excluding chromosome Y). Recent work has helped explain a large number of these unresolved regions as 'structural' in nature. Another class of gaps is likely to be refractory to clone-based approaches, and cannot be approached in ways previously described. We present an approach for closing these gaps using 454 sequencing. As a proof of principle, we closed all three remaining non-structural gaps in chromosome 15 ...[more]
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