A novel and universal method for microRNA RT-qPCR data normalization.
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ABSTRACT:
SUBMITTER: Mestdagh P
PROVIDER: S-EPMC2718498 | biostudies-literature | 2009
REPOSITORIES: biostudies-literature
Publications
A novel and universal method for microRNA RT-qPCR data normalization.
Mestdagh Pieter P Van Vlierberghe Pieter P De Weer An A Muth Daniel D Westermann Frank F Speleman Frank F Vandesompele Jo J
Genome biology 20090616 6
Gene expression analysis of microRNA molecules is becoming increasingly important. In this study we assess the use of the mean expression value of all expressed microRNAs in a given sample as a normalization factor for microRNA real-time quantitative PCR data and compare its performance to the currently adopted approach. We demonstrate that the mean expression value outperforms the current normalization strategy in terms of better reduction of technical variation and more accurate appreciation o ...[more]
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