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Structural and biophysical properties of the integrin-associated cytoskeletal protein talin.

ABSTRACT: Talin is a large cytoskeletal protein (2541 amino acid residues) which plays a key role in integrin-mediated events that are crucial for cell adhesion, migration, proliferation and survival. This review summarises recent work on the structure of talin and on some of the structurally better defined interactions with other proteins. The N-terminal talin head (approx. 50 kDa) consists of an atypical FERM domain linked to a long flexible rod (approx. 220 kDa) made up of a series of amphipathic helical bundle domains. The F3 FERM subdomain in the head binds the cytoplasmic tail of integrins, but this interaction can be inhibited by an interaction of F3 with a helical bundle in the talin rod, the so-called "autoinhibited form" of the molecule. The talin rod contains a second integrin-binding site, at least two actin-binding sites and a large number of binding sites for vinculin, which is important in reinforcing the initial integrin-actin link mediated by talin. The vinculin binding sites are defined by hydrophobic residues buried within helical bundles, and these must unfold to allow vinculin binding. Recent experiments suggest that this unfolding may be mediated by mechanical force exerted on the talin molecule by actomyosin contraction. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s12551-009-0009-4) contains supplementary material, which is available to authorized users.

SUBMITTER: Roberts GC

PROVIDER: S-EPMC2720171 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature

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Structural and biophysical properties of the integrin-associated cytoskeletal protein talin.

Roberts Gordon C K GC Critchley David R DR

Biophysical reviews 20090604 2

Talin is a large cytoskeletal protein (2541 amino acid residues) which plays a key role in integrin-mediated events that are crucial for cell adhesion, migration, proliferation and survival. This review summarises recent work on the structure of talin and on some of the structurally better defined interactions with other proteins. The N-terminal talin head (approx. 50 kDa) consists of an atypical FERM domain linked to a long flexible rod (approx. 220 kDa) made up of a series of amphipathic helic ...[more]

PMID: 19655048

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Structural and biophysical properties of the integrin-associated cytoskeletal protein talin.

Publications

Structural and biophysical properties of the integrin-associated cytoskeletal protein talin.

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