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Short actin-based mechanism for light-directed chloroplast movement in Arabidopsis.


ABSTRACT: Organelle movement is essential for proper function of living cells. In plants, these movements generally depend on actin filaments, but the underlying mechanism is unknown. Here, in Arabidopsis, we identify associations of short actin filaments along the chloroplast periphery on the plasma membrane side associated with chloroplast photorelocation and anchoring to the plasma membrane. We have termed these chloroplast-actin filaments (cp-actin filaments). Cp-actin filaments emerge from the chloroplast edge and exhibit rapid turnover. The presence of cp-actin filaments depends on an actin-binding protein, chloroplast unusual positioning1 (CHUP1), localized on the chloroplast envelope. chup1 mutant lacked cp-actin filaments but showed normal cytoplasmic actin filaments. When irradiated with blue light to induce chloroplast movement, cp-actin filaments relocalize to the leading edge of chloroplasts before and during photorelocation and are regulated by 2 phototropins, phot1 and phot2. Our findings suggest that plants evolved a unique actin-based mechanism for organelle movement.

SUBMITTER: Kadota A 

PROVIDER: S-EPMC2722281 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature

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Short actin-based mechanism for light-directed chloroplast movement in Arabidopsis.

Kadota Akeo A   Yamada Noboru N   Suetsugu Noriyuki N   Hirose Mana M   Saito Chieko C   Shoda Keiko K   Ichikawa Satoshi S   Kagawa Takatoshi T   Nakano Akihiko A   Wada Masamitsu M  

Proceedings of the National Academy of Sciences of the United States of America 20090720 31


Organelle movement is essential for proper function of living cells. In plants, these movements generally depend on actin filaments, but the underlying mechanism is unknown. Here, in Arabidopsis, we identify associations of short actin filaments along the chloroplast periphery on the plasma membrane side associated with chloroplast photorelocation and anchoring to the plasma membrane. We have termed these chloroplast-actin filaments (cp-actin filaments). Cp-actin filaments emerge from the chloro  ...[more]

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