Evaluation of binding selectivities and affinities of platinum-based quadruplex interactive complexes by electrospray ionization mass spectrometry.

ABSTRACT: The quadruplex binding affinities and selectivities of two large pi-surface Pt(II) phenanthroimidazole complexes, as well as a smaller pi-surface platinum bipyridine complex and a larger Ru(II) complex, were evaluated by electrospray ionization mass spectrometry. Circular dichroism (CD) spectroscopy was used to determine the structures of various quadruplexes and to study the thermal denaturation of the quadruplexes in the absence and presence of the metal complexes. In addition, chemical probe reactions with glyoxal were used to monitor the changes in the quadruplex conformation because of association with the complexes. The platinum phenanthroimidazole complexes show increased affinity for several of the quadruplexes with elongated loops between guanine repeats. Quadruplexes with shorter

SUBMITTER: Pierce SE 

PROVIDER: S-EPMC2725001 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature