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Plasmid-based system for high-level gene expression and antisense gene knockdown in Bartonella henselae.

ABSTRACT: Six broad-host-range plasmid vectors were developed to study gene expression in Bartonella henselae. The vectors were used to express a beta-galactosidase reporter gene in B. henselae and to generate antisense RNA for gene knockdown. When applied to ompR, a putative transcription response regulator of B. henselae, this antisense RNA gene knockdown strategy reduced bacterial invasion of human endothelial cells by over 60%.

SUBMITTER: Gillaspie D

PROVIDER: S-EPMC2725459 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature

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Plasmid-based system for high-level gene expression and antisense gene knockdown in Bartonella henselae.

Gillaspie Devin D Perkins Izabella I Larsen Kellie K McCord Amy A Pangonis Scott S Sweger Debra D Seleem Mohamed N MN Sriranganathan Nammalwar N Anderson Burt E BE

Applied and environmental microbiology 20090619 16

Six broad-host-range plasmid vectors were developed to study gene expression in Bartonella henselae. The vectors were used to express a beta-galactosidase reporter gene in B. henselae and to generate antisense RNA for gene knockdown. When applied to ompR, a putative transcription response regulator of B. henselae, this antisense RNA gene knockdown strategy reduced bacterial invasion of human endothelial cells by over 60%. ...[more]

PMID: 19542333

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