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Biochemical characterization of haloalkane dehalogenases DrbA and DmbC, Representatives of a Novel Subfamily.

ABSTRACT: This study focuses on two representatives of experimentally uncharacterized haloalkane dehalogenases from the subfamily HLD-III. We report biochemical characterization of the expression products of haloalkane dehalogenase genes drbA from Rhodopirellula baltica SH1 and dmbC from Mycobacterium bovis 5033/66. The DrbA and DmbC enzymes show highly oligomeric structures and very low activities with typical substrates of haloalkane dehalogenases.

SUBMITTER: Jesenska A

PROVIDER: S-EPMC2725514 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature

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Biochemical characterization of haloalkane dehalogenases DrbA and DmbC, Representatives of a Novel Subfamily.

Jesenská Andrea A Monincová Marta M Koudeláková Tána T Hasan Khomaini K Chaloupková Radka R Prokop Zbynek Z Geerlof Arie A Damborsky Jirí J

Applied and environmental microbiology 20090605 15

This study focuses on two representatives of experimentally uncharacterized haloalkane dehalogenases from the subfamily HLD-III. We report biochemical characterization of the expression products of haloalkane dehalogenase genes drbA from Rhodopirellula baltica SH1 and dmbC from Mycobacterium bovis 5033/66. The DrbA and DmbC enzymes show highly oligomeric structures and very low activities with typical substrates of haloalkane dehalogenases. ...[more]

PMID: 19502442

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