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Development of a multiplex real-time PCR for detection and differentiation of malignant catarrhal fever viruses in clinical samples.

ABSTRACT: A multiplex real-time PCR was developed using a single pair of primers and fluorescent probes specific for five malignant catarrhal fever viruses and an internal positive control. The assay was able to simultaneously detect and differentiate the viruses in clinical samples with high sensitivity (97.2%) and specificity (100%).

SUBMITTER: Cunha CW 

PROVIDER: S-EPMC2725674 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature

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Development of a multiplex real-time PCR for detection and differentiation of malignant catarrhal fever viruses in clinical samples.

Cunha Cristina W CW   Otto Lisa L   Taus Naomi S NS   Knowles Donald P DP   Li Hong H  

Journal of clinical microbiology 20090603 8

A multiplex real-time PCR was developed using a single pair of primers and fluorescent probes specific for five malignant catarrhal fever viruses and an internal positive control. The assay was able to simultaneously detect and differentiate the viruses in clinical samples with high sensitivity (97.2%) and specificity (100%). ...[more]

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