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Expanded multilocus sequence typing for burkholderia species.


ABSTRACT: PCR primers targeting loci in the current Burkholderia cepacia complex multilocus sequence typing scheme were redesigned to (i) more reliably amplify these loci from B. cepacia complex species, (ii) amplify these same loci from additional Burkholderia species, and (iii) enable the use of a single primer set per locus for both amplification and DNA sequencing.

SUBMITTER: Spilker T 

PROVIDER: S-EPMC2725695 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature

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Expanded multilocus sequence typing for burkholderia species.

Spilker Theodore T   Baldwin Adam A   Bumford Amy A   Dowson Chris G CG   Mahenthiralingam Eshwar E   LiPuma John J JJ  

Journal of clinical microbiology 20090603 8


PCR primers targeting loci in the current Burkholderia cepacia complex multilocus sequence typing scheme were redesigned to (i) more reliably amplify these loci from B. cepacia complex species, (ii) amplify these same loci from additional Burkholderia species, and (iii) enable the use of a single primer set per locus for both amplification and DNA sequencing. ...[more]

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