Unknown

Dataset Information

0

Trypanosoma brucei PUF9 regulates mRNAs for proteins involved in replicative processes over the cell cycle.


ABSTRACT: Many genes that are required at specific points in the cell cycle exhibit cell cycle-dependent expression. In the early-diverging model eukaryote and important human pathogen Trypanosoma brucei, regulation of gene expression in the cell cycle and other processes is almost entirely post-transcriptional. Here, we show that the T. brucei RNA-binding protein PUF9 stabilizes certain transcripts during S-phase. Target transcripts of PUF9--LIGKA, PNT1 and PNT2--were identified by affinity purification with TAP-tagged PUF9. RNAi against PUF9 caused an accumulation of cells in G2/M phase and unexpectedly destabilized the PUF9 target mRNAs, despite the fact that most known Puf-domain proteins promote degradation of their target mRNAs. The levels of the PUF9-regulated transcripts were cell cycle dependent, peaking in mid- to late- S-phase, and this effect was abolished when PUF9 was targeted by RNAi. The sequence UUGUACC was over-represented in the 3' UTRs of PUF9 targets; a point mutation in this motif abolished PUF9-dependent stabilization of a reporter transcript carrying the PNT1 3' UTR. LIGKA is involved in replication of the kinetoplast, and here we show that PNT1 is also kinetoplast-associated and its over-expression causes kinetoplast-related defects, while PNT2 is localized to the nucleus in G1 phase and redistributes to the mitotic spindle during mitosis. PUF9 targets may constitute a post-transcriptional regulon, encoding proteins involved in temporally coordinated replicative processes in early G2 phase.

SUBMITTER: Archer SK 

PROVIDER: S-EPMC2727004 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature

altmetric image

Publications

Trypanosoma brucei PUF9 regulates mRNAs for proteins involved in replicative processes over the cell cycle.

Archer Stuart K SK   Luu Van-Duc VD   de Queiroz Rafael A RA   Brems Stefanie S   Clayton Christine C  

PLoS pathogens 20090828 8


Many genes that are required at specific points in the cell cycle exhibit cell cycle-dependent expression. In the early-diverging model eukaryote and important human pathogen Trypanosoma brucei, regulation of gene expression in the cell cycle and other processes is almost entirely post-transcriptional. Here, we show that the T. brucei RNA-binding protein PUF9 stabilizes certain transcripts during S-phase. Target transcripts of PUF9--LIGKA, PNT1 and PNT2--were identified by affinity purification  ...[more]

Similar Datasets

| S-EPMC2824036 | biostudies-literature
| S-EPMC10374059 | biostudies-literature
| S-EPMC4152294 | biostudies-literature
| S-EPMC3069104 | biostudies-literature
| S-EPMC8448533 | biostudies-literature
| S-EPMC8460611 | biostudies-literature
| S-EPMC7470953 | biostudies-literature
| S-EPMC4426598 | biostudies-literature
| S-EPMC3034197 | biostudies-literature
| S-EPMC8580256 | biostudies-literature