Ontology highlight
ABSTRACT:
SUBMITTER: Yi-Brunozzi HY
PROVIDER: S-EPMC2729084 | biostudies-literature | 2008 Mar
REPOSITORIES: biostudies-literature
Yi-Brunozzi Hye Young HY Brinson Robert G RG Brabazon Danielle M DM Lener Daniela D Le Grice Stuart F J SF Marino John P JP
Chemistry & biology 20080301 3
A purine-rich region of the plus-strand RNA genome of retroviruses and long terminal repeat (LTR)-containing retrotransposons, known as the polypurine tract (PPT), is resistant to hydrolysis by the RNase H domain of reverse transcriptase (RT) and ultimately serves as a primer for plus-strand DNA synthesis. The mechanisms underlying PPT resistance and selective processing remain largely unknown. Here, two RNA/DNA hybrids derived from the PPTs of HIV-1 and Ty3 were probed using high-resolution NMR ...[more]