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C2AB: a molecular glue for lipid vesicles with a negatively charged surface.

ABSTRACT: Artificial particulate systems such as lipid vesicles are found in a variety of biomedical applications such as drug delivery and targeting. More versatile layers of control would be added if liposomes could be glued together on demand while stabilized against fusion. Here, we present a two-component molecular glue composed of a protein and calcium ions, with each component specialized for fast and specific binding to negatively charged lipid membranes. Upon mixing the two components, the high affinity binding of this glue starts to tightly bridge two lipid vesicles on a subsecond scale. Furthermore, highly charged liposomes are beneficial in preventing spontaneous fusion before applying the molecular glue.

SUBMITTER: Diao J

PROVIDER: S-EPMC2730783 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature

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C2AB: a molecular glue for lipid vesicles with a negatively charged surface.

Diao Jiajie J Yoon Tae-Young TY Su Zengliu Z Shin Yeon-Kyun YK Ha Taekjip T

Langmuir : the ACS journal of surfaces and colloids 20090701 13

Artificial particulate systems such as lipid vesicles are found in a variety of biomedical applications such as drug delivery and targeting. More versatile layers of control would be added if liposomes could be glued together on demand while stabilized against fusion. Here, we present a two-component molecular glue composed of a protein and calcium ions, with each component specialized for fast and specific binding to negatively charged lipid membranes. Upon mixing the two components, the high a ...[more]

PMID: 19563216

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