Rhythm changes of clock genes, apoptosis-related genes and atherosclerosis-related genes in apolipoprotein E knockout mice.

ABSTRACT:

Background

Acute myocardial infarction and stroke occur more frequently in the morning, suggesting a role of the circadian clock in these main causes of death, secondary to atherosclerosis.

Objectives

To investigate the expression of clock genes, apoptosis-related genes and atherosclerosis-related genes in the process of atherosclerosis.

Methods

Apolipoprotein E knockout (ApoE-/-) mice were used to establish animal models of early and advanced atherosclerosis. Real-time polymerase chain reaction, Western blotting and microarray assays were used to detect the expression of clock genes, apoptosis-related genes and atherosclerosis-related genes.

Results

Clock genes in ApoE-/- and C57BL/6J mouse hearts exhibited daily oscillations at the messenger RNA level. Howeve

SUBMITTER: Xu C 

PROVIDER: S-EPMC2732375 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature