Ontology highlight
ABSTRACT:
SUBMITTER: Mennella V
PROVIDER: S-EPMC2733746 | biostudies-literature | 2009 Aug
REPOSITORIES: biostudies-literature
Mennella Vito V Tan Dong-Yan DY Buster Daniel W DW Asenjo Ana B AB Rath Uttama U Ma Ao A Sosa Hernando J HJ Sharp David J DJ
The Journal of cell biology 20090817 4
Microtubule (MT)-destabilizing kinesin 13s perform fundamental roles throughout the cell cycle. In this study, we show that the Drosophila melanogaster kinesin 13, KLP10A, is phosphorylated in vivo at a conserved serine (S573) positioned within the alpha-helix 5 of the motor domain. In vitro, a phosphomimic KLP10A S573E mutant displays a reduced capacity to depolymerize MTs but normal affinity for the MT lattice. In cells, replacement of endogenous KLP10A with KLP10A S573E dampens MT plus end dy ...[more]