Optically modulated fluorophores for selective fluorescence signal recovery.
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ABSTRACT:
SUBMITTER: Richards CI
PROVIDER: S-EPMC2734916 | biostudies-literature | 2009 Apr
REPOSITORIES: biostudies-literature
Publications
Optically modulated fluorophores for selective fluorescence signal recovery.
Richards Chris I CI Hsiang Jung-Cheng JC Senapati Dulal D Patel Sandeep S Yu Junhua J Vosch Tom T Dickson Robert M RM
Journal of the American Chemical Society 20090401 13
Fluorescence imaging in biological sciences is hindered by significant depth-dependent signal attenuation and highly fluorescent backgrounds. We have developed optically modulated near-IR-emitting few-atom Ag nanodots that are selectively and dynamically photobrightened upon simultaneous excitation with a secondary laser, enabling high-sensitivity image extraction to reveal only the demodulated fluorophores. Image demodulation is demonstrated in high-background environments to extract weak signa ...[more]
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