Ontology highlight
ABSTRACT:
SUBMITTER: Zhang YW
PROVIDER: S-EPMC2736145 | biostudies-literature | 2009 Aug
REPOSITORIES: biostudies-literature
Zhang You-Wei YW Brognard John J Coughlin Chris C You Zhongsheng Z Dolled-Filhart Marisa M Aslanian Aaron A Manning Gerard G Abraham Robert T RT Hunter Tony T
Molecular cell 20090801 4
ATR and Chk1 are two key protein kinases in the replication checkpoint. Activation of ATR-Chk1 has been extensively investigated, but checkpoint termination and replication fork restart are less well understood. Here, we report that DNA damage not only activates Chk1, but also exposes a degron-like region at the carboxyl terminus of Chk1 to an Fbx6-containing SCF (Skp1-Cul1-F box) E3 ligase, which mediates the ubiquitination and degradation of Chk1 and, in turn, terminates the checkpoint. The pr ...[more]