Ontology highlight
ABSTRACT:
SUBMITTER: Smith KE
PROVIDER: S-EPMC2737235 | biostudies-literature | 2009 Jun
REPOSITORIES: biostudies-literature
Smith Kristin E KE Linser Paul J PJ
Journal of RNAi and gene silencing : an international journal of RNA and gene targeting research 20090617 1
RNAi has been used extensively to down-regulate proteins in adult mosquitoes; however, it is not well adapted for use in larvae. Larval mosquitoes can generate a pH as high as 10.5 in the anterior region of their midgut. The mechanisms responsible for the generation and maintenance of this pH are not entirely understood, but members of the carbonic anhydrase (CA) family of enzymes have been implicated. Here we use an An. gambiae larval cell line, Ag55 cells, to demonstrate that application of fu ...[more]