SPIDER image processing for single-particle reconstruction of biological macromolecules from electron micrographs.
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ABSTRACT:
SUBMITTER: Shaikh TR
PROVIDER: S-EPMC2737740 | biostudies-literature | 2008
REPOSITORIES: biostudies-literature
Publications
SPIDER image processing for single-particle reconstruction of biological macromolecules from electron micrographs.
Shaikh Tanvir R TR Gao Haixiao H Baxter William T WT Asturias Francisco J FJ Boisset Nicolas N Leith Ardean A Frank Joachim J
Nature protocols 20080101 12
This protocol describes the reconstruction of biological molecules from the electron micrographs of single particles. Computation here is performed using the image-processing software SPIDER and can be managed using a graphical user interface, termed the SPIDER Reconstruction Engine. Two approaches are described to obtain an initial reconstruction: random-conical tilt and common lines. Once an existing model is available, reference-based alignment can be used, a procedure that can be iterated. A ...[more]
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