Differentiating proteomic biomarkers in breast cancer by laser capture microdissection and MALDI MS.
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ABSTRACT:
SUBMITTER: Sanders ME
PROVIDER: S-EPMC2738605 | biostudies-literature | 2008 Apr
REPOSITORIES: biostudies-literature
Publications
Differentiating proteomic biomarkers in breast cancer by laser capture microdissection and MALDI MS.
Sanders Melinda E ME Dias Eduardo C EC Xu Baogang J BJ Mobley James A JA Billheimer Dean D Roder Heinrich H Grigorieva Julia J Dowsett Mitchell M Arteaga Carlos L CL Caprioli Richard M RM
Journal of proteome research 20080404 4
We assessed proteomic patterns in breast cancer using MALDI MS and laser capture microdissected cells. Protein and peptide expression in invasive mammary carcinoma versus normal mammary epithelium and estrogen-receptor positive versus estrogen-receptor negative tumors were compared. Biomarker candidates were identified by statistical analysis and classifiers were developed and validated in blinded test sets. Several of the m/ z features used in the classifiers were identified by LC-MS/MS and two ...[more]
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