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ABSTRACT:
SUBMITTER: Lewandowski JR
PROVIDER: S-EPMC2738631 | biostudies-literature | 2009 Jul
REPOSITORIES: biostudies-literature
Lewandowski Józef R JR De Paëpe Gaël G Eddy Matthew T MT Struppe Jochem J Maas Werner W Griffin Robert G RG
The journal of physical chemistry. B 20090701 27
We demonstrate the successful application of (13)C-(13)C proton assisted recoupling (PAR) on [U-(13)C,(15)N] N-f-MLF-OH and [U-(13)C,(15)N] protein GB1 at high magic angle spinning (MAS) frequencies (omega(r)/2pi = 65 kHz). Specifically, by combining PAR mixing with low power heteronuclear decoupling (omega(1H)/2pi approximately 16 kHz) and high spinning frequencies, we obtain high resolution 2D spectra displaying long-range (13)C-(13)C contacts from which distance estimates can be extracted. Th ...[more]