Ontology highlight
ABSTRACT:
SUBMITTER: Xue H
PROVIDER: S-EPMC2741170 | biostudies-literature | 2009 Aug
REPOSITORIES: biostudies-literature
Xue Haipeng H Wu Sen S Papadeas Sophia T ST Spusta Steve S Swistowska Anna Maria AM MacArthur Chad C CC Mattson Mark P MP Maragakis Nicholas J NJ Capecchi Mario R MR Rao Mahendra S MS Zeng Xianmin X Liu Ying Y
Stem cells (Dayton, Ohio) 20090801 8
In this study, we targeted Olig2, a basic helix-loop-helix transcription factor that plays an important role in motoneuron and oligodendrocyte development, in human embryonic stem cell (hESC) line BG01 by homologous recombination. One allele of Olig2 locus was replaced by a green fluorescent protein (GFP) cassette with a targeting efficiency of 5.7%. Targeted clone R-Olig2 (like the other clones) retained pluripotency, typical hESC morphology, and a normal parental karyotype 46,XY. Most importan ...[more]