A protein-based set of reference markers for liver tissues and hepatocellular carcinoma.

ABSTRACT:

Background

During the last decade, investigations have focused on revealing genes or proteins that are involved in HCC carcinogenesis using either genetic or proteomic techniques. However, these studies are overshadowed by a lack of good internal reference standards. The need to identify "housekeeping" markers, whose expression is stable in various experimental and clinical conditions, is therefore of the utmost clinical relevance in quantitative studies. This is the first study employed 2-DE analysis to screen for potential reference markers and aims to correlate the abundance of these proteins with their level of transcript expression.

Methods

A Chinese cohort of 224 liver tissues samples (105 cancerous, 103 non-tumourous cirrhotic, and 16 normal) was profiled using 2-DE a

SUBMITTER: Sun S 

PROVIDER: S-EPMC2742551 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature