Monocyte activation by necrotic cells is promoted by mitochondrial proteins and formyl peptide receptors.

ABSTRACT:

Objective

Necrotic cells evoke potent innate immune responses through unclear mechanisms. The mitochondrial fraction of the cell retains constituents of its bacterial ancestors, including N-formyl peptides, which are potentially immunogenic. Thus, we hypothesized that the mitochondrial fraction of the cell, particularly N-formyl peptides, contributes significantly to the activation of monocytes by necrotic cells.

Design

Human peripheral blood monocytes were incubated with necrotic cell fractions and mitochondrial proteins to investigate their potential for immune cell activation.

Setting

University Medical Center Research Laboratory.

Subjects

Healthy human adults served as blood donors.

Measurements and main results

Human blood monocyte activation was me

SUBMITTER: Crouser ED 

PROVIDER: S-EPMC2743203 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature