Ontology highlight
ABSTRACT:
SUBMITTER: Schweppe JJ
PROVIDER: S-EPMC2743985 | biostudies-literature | 2009 Jun-Aug
REPOSITORIES: biostudies-literature
Schweppe James J JJ Jain Chaitanya C White Susan A SA
Biochimica et biophysica acta 20090519 6-8
The S. cerevisiae ribosomal protein L30e is an autoregulatory protein that binds to its own pre-mRNA and mature mRNA to inhibit splicing and translation, respectively. The L30e RNA-binding element is a stem-asymmetric loop-stem that forms a kink-turn. A bacterial genetic system was designed to test the ability of protein variants to repress the expression of reporter mRNAs containing the L30e RNA-binding element. Initial screens revealed that changes in several RNA nucleotides had a measurable e ...[more]