Ontology highlight
ABSTRACT:
SUBMITTER: Lopez-Ferrer D
PROVIDER: S-EPMC2744207 | biostudies-literature | 2008 Sep
REPOSITORIES: biostudies-literature
López-Ferrer Daniel D Heibeck Tyler H TH Petritis Konstantinos K Hixson Kim K KK Qian Weijun W Monroe Matthew E ME Mayampurath Anoop A Moore Ronald J RJ Belov Mikhail E ME Camp David G DG Smith Richard D RD
Journal of proteome research 20080808 9
A new sample processing workflow that uses high intensity focused ultrasound to rapidly reduce and alkylate cysteines, digest proteins and then label peptides with (18)O was developed for quantitative proteomics applications. Each step was individually refined to minimize reaction times, peptide loses and undesired byproducts or modifications. When this novel workflow was used, mouse plasma proteins were successfully denatured, alkylated, in-solution digested, and (18)O-labeled in <10 min for su ...[more]