Ontology highlight
ABSTRACT:
SUBMITTER: Kandavelou K
PROVIDER: S-EPMC2744961 | biostudies-literature | 2009 Oct
REPOSITORIES: biostudies-literature
Kandavelou Karthikeyan K Ramalingam Sivaprakash S London Viktoriya V Mani Mala M Wu Joy J Alexeev Vitali V Civin Curt I CI Chandrasegaran Srinivasan S
Biochemical and biophysical research communications 20090725 1
Targeted introduction of a double-stranded break (DSB) using designer zinc finger nucleases (ZFNs) in mammalian cells greatly enhances gene targeting - homologous recombination (HR) at a chosen endogenous target gene, which otherwise is limited by low spontaneous rate of HR. Here, we report that efficient ZFN-mediated gene correction occurs at a transduced, transcriptionally active, mutant GFP locus by homology-directed repair, and that efficient mutagenesis by non-homologous end joining (NHEJ) ...[more]