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Biogenesis of bacterial membrane vesicles.

ABSTRACT: Membrane vesicle (MV) release remains undefined, despite its conservation among replicating Gram-negative bacteria both in vitro and in vivo. Proteins identified in Salmonella MVs, derived from the envelope, control MV production via specific defined domains that promote outer membrane protein-peptidoglycan (OM-PG) and OM protein-inner membrane protein (OM-PG-IM) interactions within the envelope structure. Modulation of OM-PG and OM-PG-IM interactions along the cell body and at division septa, respectively, maintains membrane integrity while co-ordinating localized release of MVs with distinct size distribution and protein content. These data support a model of MV biogenesis, wherein bacterial growth and division invoke temporary, localized reductions in the density of OM-PG and OM-PG-IM associations within the envelope structure, thus releasing OM as MVs.

SUBMITTER: Deatherage BL

PROVIDER: S-EPMC2745257 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature

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Biogenesis of bacterial membrane vesicles.

Deatherage Brooke L BL Lara J Cano JC Bergsbaken Tessa T Rassoulian Barrett Sara L SL Lara Stephanie S Cookson Brad T BT

Molecular microbiology 20090508 6

Membrane vesicle (MV) release remains undefined, despite its conservation among replicating Gram-negative bacteria both in vitro and in vivo. Proteins identified in Salmonella MVs, derived from the envelope, control MV production via specific defined domains that promote outer membrane protein-peptidoglycan (OM-PG) and OM protein-inner membrane protein (OM-PG-IM) interactions within the envelope structure. Modulation of OM-PG and OM-PG-IM interactions along the cell body and at division septa, r ...[more]

PMID: 19432795

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