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Repair of DNA lesions associated with triplex-forming oligonucleotides.

ABSTRACT: Triplex-forming oligonucleotides (TFOs) are gene targeting tools that can bind in the major groove of duplex DNA in a sequence-specific manner. When bound to DNA, TFOs can inhibit gene expression, can position DNA-reactive agents to specific locations in the genome, or can induce targeted mutagenesis and recombination. There is evidence that third strand binding, alone or with an associated cross-link, is recognized and metabolized by DNA repair factors, particularly the nucleotide excision repair pathway. This review examines the evidence for DNA repair of triplex-associated lesions.

SUBMITTER: Chin JY

PROVIDER: S-EPMC2745299 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature

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Repair of DNA lesions associated with triplex-forming oligonucleotides.

Chin Joanna Y JY Glazer Peter M PM

Molecular carcinogenesis 20090401 4

Triplex-forming oligonucleotides (TFOs) are gene targeting tools that can bind in the major groove of duplex DNA in a sequence-specific manner. When bound to DNA, TFOs can inhibit gene expression, can position DNA-reactive agents to specific locations in the genome, or can induce targeted mutagenesis and recombination. There is evidence that third strand binding, alone or with an associated cross-link, is recognized and metabolized by DNA repair factors, particularly the nucleotide excision repa ...[more]

PMID: 19072762

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