Ontology highlight
ABSTRACT:
SUBMITTER: Chen HH
PROVIDER: S-EPMC2746678 | biostudies-literature | 2009 Apr
REPOSITORIES: biostudies-literature
Chen Hunter H HH Ho Yi-Ping YP Jiang Xuan X Mao Hai-Quan HQ Wang Tza-Huei TH Leong Kam W KW
Nano today 20090401 2
Nanoscale vectors comprised of cationic polymers that condense DNA to form nanocomplexes are promising options for gene transfer. The rational design of more efficient nonviral gene carriers will be possible only with better mechanistic understanding of the critical rate-limiting steps, such as nanocomplex unpacking to release DNA and degradation by nucleases. We present a two-step quantum dot fluorescence resonance energy transfer (two-step QD-FRET) approach to simultaneously and non-invasively ...[more]