A novel analytical method for in vivo phosphate tracking.
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ABSTRACT:
SUBMITTER: Gu H
PROVIDER: S-EPMC2748124 | biostudies-literature | 2006 Oct
REPOSITORIES: biostudies-literature
Publications
A novel analytical method for in vivo phosphate tracking.
Gu Hong H Lalonde Sylvie S Okumoto Sakiko S Looger Loren L LL Scharff-Poulsen Anne Marie AM Grossman Arthur R AR Kossmann Jens J Jakobsen Iver I Frommer Wolf B WB
FEBS letters 20061002 25
Genetically-encoded fluorescence resonance energy transfer (FRET) sensors for phosphate (P(i)) (FLIPPi) were engineered by fusing a predicted Synechococcus phosphate-binding protein (PiBP) to eCFP and Venus. Purified fluorescent indicator protein for inorganic phosphate (FLIPPi), in which the fluorophores are attached to the same PiBP lobe, shows P(i)-dependent increases in FRET efficiency. FLIPPi affinity mutants cover P(i) changes over eight orders of magnitude. COS-7 cells co-expressing a low ...[more]
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