Transcriptional profiling of mRNA expression in the mouse distal colon.

ABSTRACT:

Background & aims

Intestinal epithelial cells and the myenteric plexus of the mouse gastrointestinal tract contain a circadian clock-based intrinsic time-keeping system. Because disruption of the biological clock has been associated with increased susceptibility to colon cancer and gastrointestinal symptoms, we aimed to identify rhythmically expressed genes in the mouse distal colon.

Methods

Microarray analysis was used to identify genes that were rhythmically expressed over a 24-hour light/dark cycle. The transcripts were then classified according to expression pattern, function, and association with physiologic and pathophysiologic processes of the colon.

Results

A circadian gene expression pattern was detected in approximately 3.7% of distal colonic genes. A large

SUBMITTER: Hoogerwerf WA 

PROVIDER: S-EPMC2748881 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature